Other specimens in addition to the cheek cells and elodea leaves, you instructor may require you to identify several other kinds of specimens on a lab practical exam. Only lens paper will be used to wipe clean the lenses and other glass portions of the microscope. Microscopy i, an introduction to the compound light microscope revised, spring 2012 page 3 4. Three 1 g aliquots of soil were taken from each seived subsample to prepare slides for direct microscopic examination of fungal hyphae. Introducing geometric optics through experimental observations. Halogen provides a bright white that is suitable for most applications. Although microscopes comes with various type of models and applications such as. However, there will be a quiz on microscope care at the beginning of lab.
Arm objective lenses swans xooox conyrols objective mechanical sraor connss rocus iris diaphragm fxu. Introduction to the microscope lab activity part i. A confocal microscope creates sharp images of a speci men that. Q8 if the 10x objective is used with a 15x eyepiece, calculate the overall magnification of the microscope. Laboratory exercise graduate course in scanning electron microscopy sem purpose the objectives of the exercises are to demonstrate. Introduction to the microscope lab activity introduction. Materials list the materials you used to complete this lab. Use the following information to complete the letter e lab report. On august 20, a lab was started doing different test involving blood. The compound light microscope is an instrument containing two lenses, which magnifies, and a variety of knobs to resolve focus. The solution to overcome this problem is by observing them using microscope.
Magnification the degree to which the image of a specimen is enlarged resolution the ability to distinguish two objects as separate and distinct contrast the ability to see specimen detail against its background. Compound microscope lab report free download as word doc. What we perceive as color is related to the different wavelengths of light. I believe after you make a wet mount slide you use the microscope to i know this because procedure. Objective 4x scanning 10x low power 40x high dry power 100x oil immersion 4. Because the sheer amount of data makes manual annotation infeasible, data. This laboratory is designed to be the first part of your handson sem operators manual. Sections are now floated out on the surface of warm water in a flotation bath to flatten them and then picked up onto microscope slides. These two differ in many aspects and both have their own space in lab. Samples were returned to the lab, sieved 5 mm sieve, and each was divided into three subsamples. Materials characterization laboratory reports including both simple ones for better microscope. Draw picture s of the elodea cells on your report sheet for both low and high power. They examine a slide with the letter e, common things, like dust, and a prepared slide with threads to illustrate depth perception.
Microscope lab using the microscope and slide preparation micro refers to tiny, scope refers to view or look at. Laboratory exercise graduate course in scanning electron. I can understand and explain the three parts of cell theory. Model microscope thin lenses while hardly a commercial instrument, much can be learned by constructing a. Recognize and using the lab report has a jul 19, videos, lab manual. A microscope is an instrument used to see objects that are too small to be seen by the naked. So, when comes to observing them through our naked eyes are absolutely impossible. The binocular compound light microscope is used to magnify an image and allows us to study the structure of tiny cells. In this lab module, you will learn how scientists perform experiments. After you make a wet mount slide, how do you use the parts of the microscope to find specimens on low medium and high power. Introduction to the microscope lab activity introduction micro refers to tiny, scope refers to view or look at.
Lab coat face shield respirator rubber apron safety shower. Using a toothpick gently scrape the inside of your cheek to obtain epithelial cells. In this lab, various objects were examined under a microscope to see what the microscope would reveal that wasnt visible to the naked eye. The microscopes in our lab may vary from the one in the image above. Place the cheek cells into the iki on the slide and mix. Define the function of the following parts of a compound light microscope. All slides were made within 36 h of soil collection. This free teachers printable is easy to download in pdf or doc format, customize doc version only, and print with teachers printables in pdf format, you can download and print attendance charts, lesson plan forms, school calendars, and more requires an application that can read pdf files. Spinal cord and peripheral nervous system pt 3 15 terms. Lab 1 use of the microscope francis marion university.
Microscopes are used to make more detailed observations and measurements of objects too small for the naked eye. Pdf laboratory exercises for microbiology biology 552. In your lab data section, create a table with the part names and functions. Microscope characterization and fourierplane imaging. What is the difference between the fine and coarse adjustments. Ask your instructor or the lab assistant to check your hypothesis and prediction, and to make suggestions about improving your experiment. Microscope lab for freshman level biology where students learn to focus a light microscope. This procedure is discussed in the introduction to the lab manual. Only advanced users will see the difference so the choice is a personal one. Pdf 2 overview biol 552 has a challenging goal, which is to give students practical experience and. Inspect the zaf setup header to ensure that these parameters are selected. Cut out the letter e and place it on the slide face up.
The compound light microscope is an instrument containing two lenses, which magnify, and a variety of knobs to resolve focus the image. Write a report for this experiment to be handed in at the start of next weeks lab. Learn vocabulary, terms, and more with flashcards, games, and other study tools. This material is adapted from the applied microbiology laboratory manual by cynthia schauer. Lab report on microscopy michael goerz, anton haase 14. Pdf a guide on laboratory microscope with camera facilities. Lab 3 use of the microscope introduction in this laboratory you will be learning how to use one of the most important tools in biology the compound light microscope to view a variety of specimens. As a result, the microscope simulation had no effect on laboratory achievements and laboratory reports but help to develop ability to use. Fushitani 1 introduction general laws of lenses the laws describing microscopes or similar optical devices result from the laws of a single lens. Protozoans are unicellular eukaryotes with either plant or animallike characteristics. Microscopes are tools used to enlarge images of small objects so as they can be studied.
Advances through aberrationcorrected electron microscopy pdf. Proper use of microscopes requires the basic knowledge about their component parts, their resolution and proper handling. Minimum information guidelines for fluorescence microscopy arxiv. Opti 202l geometrical and instrumental optics lab 95 q7 if the 10x objective is used with a 10x eyepiece, calculate the overall magnification of the microscope.
Why is locating an object more difficult if you start with the higher power objectives than with the scanning objective. Lab report on microscope professional custom writing assistance. Do not overtighten clampsif you are unsure, ask for help. Record the absorbed specimen current with the picoammeter. What advantage does the use of the oilimmersion objective lenses provide when. Introduction to the microscope and comparison of sizes and. Through careful observation, we analyzed various protozoan cultures in order to identify characteristics associated with cell.
The lab report assistant is simply a summary of the experiments questions, diagrams if needed, and data tables that should be addressed in a formal lab report. Version 4200900001 lab report assistant this document is not meant to be a substitute for a formal laboratory report. In this lab, you will become familiar with the use of the microscope particularly oil immersion. To view your own human cheek cells under the microscope. Peal the cucumber and cut the cucumber in 4 slices of approximately. The first thing we examined under the microscope was the letter o written in pen. Introduction to the microscope lab biology junction. Using the materials available, carry out your experiment. Biolab 3 lab report 3 microscope and the cell student name. A ribbon of sections being cut from a paraffin block using a rotary microtome.
It may provide a dynamic image as with optical instruments or. Condenser the light rays are concentrated on the object to be observed by the condenser 2. Halogen or led illumination is standard on most good quality microscopes. Add one or two drops of iki solution to the central region of a clean glass slide. For example, light of 650 nm wavelength is seen as red, whereas 450 nm is seen as blue. Lab 1 use of the microscope introduction individual microbial cells are usually too small to see with the unaided, naked eye. Choose a beam current that gives a dead time of 1015% on the alloy. Examination of protozoan cultures to determine cellular structure and motion pattern abstract.
You will also use a slightly different type of light microscope called a stereoscopic dissecting microscope. Electron microscope confer some advantages over the light microscopes. Microscope, instrument that produces enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis. The objective of the experiment was to test white blood cells wbc, red blood cells rbc, platelet counts, differential of wbcs, a hematocrit, a hemoglobin analysis, and also various calculated indices. Pdf determining the effects of microscope simulation on. There are different types of microscopes that can be used in lab according to the purpose. Before coming to lab read this laboratory exercise and pp.
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